Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. J Control Release. Data File Dextran. GPC Symp. Pavlov, G.M. Molecular weight, molecular weight distribution and molecular size of a native dextran. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. (1987). Journal of Chemical Education, 80(9), 1036-1038. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). (2008). WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) This site needs JavaScript to work properly. Rheology of dextran solutions. Chem. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), (2007). WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior. ; Bataille, B. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. 8600 Rockville Pike For ellipsoids, as studied by Simha, (a/b) is a function of axial ratio. Part of The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Colloid Polym. Soc., 21, 1, 78-86. Federal government websites often end in .gov or .mil. After 24h, the cumulative permeation of FITC-dextran M.W. Journal of Colloid Science, 31, 308-328. Springer Nature. Carbohydrate Polymers, 39, 377-380. Provided by the Springer Nature SharedIt content-sharing initiative. The basic setup of a DLS instrument is shown in Figure 1. Stenekes, R.J.H. Journal of American Chemical Society, 64(11), 2716-2718. Search Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Huggins equation [41] gives the relation to the relative viscosity increase (i). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. (2003). B 113, 8020-8025. European. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Accessibility Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Formul. They concluded that a power law behavior seems to be approached only in the small molecular weight region. Intrinsic viscosity-molecular weight relationship for chitosan. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). CAS Probiotics Antimicrob Proteins. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. Chem. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. 2008;28(5):64557. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Determination of polymer structure by Gel Permeation Chromatography. Carbohydrate Research, 160, 205-214. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. Curvale, R.; Masuelli, M.; Perez Padilla, A. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. Carbohydrate Research, 340, 811-826. Molecular weight determination of polysaccharides. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. European Polymer Journal, 36, 2143-2146. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. 2023 BioMed Central Ltd unless otherwise stated. The mechanism of the dextran-induced red blood cell Aggregation. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. ; Picout, D.R. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Epub 2022 Feb 2. (2013). The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was and transmitted securely. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. In fact, short chains do contain minor branching points. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. Dextrans in Aqueous Solution. Polysaccharides-based nanoparticles as drug delivery systems. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. where tr is the column retention time in minutes (2 of 0.9792). European Biophysical Journal, 36, 85-94. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Carbohydrate Polymers 88 373-381. Transdermal drug delivery: overcoming the skins barrier function. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. These studies were carried out at the Hisko (Gunpo, Korea). Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Harding, S. E., & Simpkin, N. J. (2004). Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Garcia de la Torre, J.; Carrasco, B. 1905/05) for their financial support. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). ; Ghosh, T.K. It is well tolerated on parenteral infusion by most people but a very low incidence We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. J. Braz. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Solvent effects on polysaccharide conformation. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Carbohydrate Polymers 95 360-365. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. International Journal of Biological Macromolecules, 42, 133-137. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Microporation was performed 2, 5, and 10 times on each sample. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Human skins (Full-thickness human skin) were supplied by HansBiomed. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Sung-Wook Choi. California Privacy Statement, Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). The Mark-Houwink. Food Sci Biotechnol. On single point determination of intrinsic viscosity. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. Webdextran hydrodynamic radius. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Arond L.H. (2013). WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. European Polymer Journal, 37, 619-622. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with ; Rani, K.Y. Analytical Chemistry. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. FITC-dextran solution was prepared at 1mM in PBS. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. Hydrodynamic radii ( RH) were determined by dynamic light scattering. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly Manage cookies/Do not sell my data we use in the preference centre. 1994, 95-0355. Hennink, W.E. The Viscosity of Dilute Solutions of Long-Chain Molecules. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. Expert Rev Med Devices. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Journal of Applied Polymer Science, 27, 795-810. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer.
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